Rationale:

To continue practicing genome annotation with DNA Master by annotating two genes of Elesar’s genome.

Procedures:

1. Used an already opened and auto-annotated copy of Elesars genome on DNA Master.
2. Annotated genes 48 and 49 of Elesar.

Results:

Gene 48: SSC: [34620, 35063], CP: yes, SCS: Both, BLAST-Start: Elesar, gp45, PhagesDB, Query 1 to Subject 1, 100%, 6e-83 Gap: 113bp gap, LO: yes, RBS: Kibler7, Karlin Medium, 2.611, -3.929, no

Gene 49: SSC: [35215, 36054], CP:yes, SCS: Both, BLAST-Start: Nandita, NCBI, Query 1 to Subject 1, 95%, 8e-165, Gap: 151bp gap, LO: yes, RBS: Kibler7, Karlin Medium, 2.970, -3.344, yes

Conclusions:

From the results gathered it can be concluded that I learned more about how to annotate genomes on DNA Master.

Next Steps:

The next step will be to continue practicing with DNA Master in annotating Elesar’s genome in order to eventually annotate the genome of the phage NapoleonB.