January
28
DNA Day 3
28 January 2019 ✷ DNA Master + BLAST
Rationale: DNA from Elesar gene 1 was BLASTed in order to see if other genes exist in the NCBI database.
Procedure
- The FASTa file for Elesar was opened and autoannotated.
- The protein sequence for gene 1 was opened and copied and pasted into the BLASTp tool on NCBI’s website and run to find matching sequences,
Results
Conclusion
The NCBI BLASTp result was not very effective in locating a similar gene for Elesar Gene 1. This just means that similar genes are not stored in the database as of yet. The E value is really high for each of the results and this means that it is very likely the results are random. The identity percentages are low as well and thus the protein sequences are not very closely related.
Future plans
This same method will be applied to Napoleon B, a phage isolated in 2018, in order to analyze its genome.