Rationale:

To continue practice annotation of Elesar by becoming more familiar with how to BLAST.

Procedure:

1. Using an already open and auto-annotated Elesar Mastering DNA window, a BLAST search of gene 1 was conducted using the in program BLAST search feature.
2. A BLAST protein search for gene 1’s protein sequence was also conducted using the NCBI BLAST program.
3. Step 2 was repeated for the PhagesDB BLAST program.
4. Analyzed Results.

Results:

For both NCBI and the DNA Master BLAST searches, all hits had scores less than 40. For the Phages DB BLAST there was a hit with a score of 178 and an E value of 2e-45, with the hit being the Elesar gene 1 unknown protein.

Conclusions:

Based upon the results it can be concluded that the protein for gene 1 is unknown as shown by the BLAST result from Phages DB, considering that both the sequence blasted and the hit were actually the same genes from Elesar.

Next Steps:

I will continue practicing genome annotation via DNA Master using the phage Elesar’s genome in order to eventually annotate the genome of the phage Napoleon B.