Rationale: In order to learn how to annotate a genome I practiced annotating the first gene of Elesar.

Tools: NCBI BLAST, DNAMaster

Procedure:

1. Began by auto-annotating the genome of Elesar using the template set-up from last lab.
2. Opened the frames view and looked at the open reading frame of gene 1. Due to there being a larger Open Reading Frame(ORF) possible I moved the ORF to the Largest Open Reading Frame(LORF).
3. Noted in the annotation the Start and Stop Coordinates(SSC), the Coding Potential(CP), and Start Choice Source(SCS).
4. Blasted the product using the NCBI database, which turned up no good matches, which I noted under Blast-Start.
5. Noted the fact that this is the first gene for GAP, and noted that this is indeed the LORF.
6. Opened up frames and highlighted the ORF and clicked RBS. Using a Kibler7 Scoring Matrix and a Karlin Medium Spacing Matrix, I recorded the Z Score, Final Score, and whether or not it was the best Final Score.
7. Posted and saved file.

Results:

Results of RBS.

Conclusion and Future Work: I’ve learned the first few steps to annotating a genome. Now I will begin working on annotating genes by myself.