Rationale:

to increase focus on certain genes and learn to annotate genes

Procedure:

1. open  DNA master
2. Preferences >Local settings >New Features
3.  Type the following template command in the the “template to be installed box” :” SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn .
4. check the “insert template into notes during Auto Annotation” box. Then Apply>OK
5. Open FastA file for elesar using the following steps, File>open>FastA multiple sequence file> Choose Elesar.FastA
6. click export on window with the extracted file and then choose Create sequence from this entry only.
7.  to see the frames, go to DNA> Frames.
8. then click ORF on the new window to see the highlighted genes.
9. this is where time for lab ended.

Results:

auto annotation identified in 65 ORFs, of which 12 were reverse ORFS and 53 were forward ORFs, of which ORF 19 waas the longest.

Conclusion:

Reverse ORFs seem to be the most uncommon in the genome. more interpretation may be later required due to lack of porper knowledge as of now.

Future Steps:

the next lab will involve more work identifying genes and find gaps and overlaps between the auto annotated genes. It may involve identification of possible things that autoannotation missed.