Rationale:

The purpose of this lab was to further understand DNA Master and the information presented for the future.

Tools:

• Computational Device
• DNA Master program
• Elesar FASTA File

Results:

• After the presentation about Ebola was given, DNA Master has been opened and the phage Elesar FASTA file was uploaded.
• The file was then exported in order for the sequence to be created.
• After it was exported, it was auto-annotated from the option of genome then auto-annotate.
• After annotation, the information under Features was explained such as gap and overlap calculations and practice was done with gene 19.
• Then, the frames were explained and purpose for their different structures along with the purpose of six reading frames.
• The ORF’s were viewed and future tools were discussed.

Conclusion:

From this lab, DNA Master was explained into more detail such as which preferences were needed and which steps needed to be taken to view the open reading frames. Future plans were explained and which tools were needed to accomplish those tasks. We also practiced gap and overlap calculations in order for future research.  Along with the calculations, the 5′ to 3′ for each base pair was explained and they were supposed to be read.

Future Work:

In the next lab, DNA Master will further be explained and actual annotation will begin.