Rationale:

The purpose was to learn about the features of DNA Master specifically about open reading frames using Elesar.  

Tools/Procedure:

1. DNA Master was opened and the code, accessed through file then preference then local settings then new feature, was changed to “SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn”.  
2. Elesar was added to DNA Master and Auto Annotated.  
3. The ORF’s of Elesar were observed.  

Results: 

Auto Annotation of Elesar using a Fasta File was obtained using DNA Master. Reverse ORF’s represented by the highlighted portions were viewed.  

Conclusion: 

More features of DNA Master were learnt specifically about ORF’s. Concepts such as reading frames, gaps and overlaps were learnt as well.

Future Work:  

To acquire more knowledge about DNA Master’s features and understand the data presented for Elesar which will aid when annotating NapoleanB.