Rationale

Today we will learn more about reading frames and explore more of DNA Master using the genome of Elesar.

Procedure

• DNA Master was opened and the template “SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST:SIF-HHPred: SIF-Syn” was uploaded for future annotations.
• Elesar was opened using the FastA file and the new template was employed to autoannotate.
• ORFs were also accessed to be observed and begin the process of learning their functions.

Results

More information on gaps and overlaps, as well as ORFs were presented. Knowledge about DNA Master and ORFs are still limited, therefore no notable results were achieved from the day. The new template may be used for future autoannotations.  A better understanding of DNA Master and the different tools in the program was reached instead.

Conclusion/Future Steps

The tools being used in DNA Master will allow for the autoannotation of NapoleonB in the future. DNA Master will be explored more using Elesar to become familiar with the program.