1/23/19

Rational:

To further annotate the genome of Elesar and starting to check for mistakes in the auto-annotation as well as learn how to calculate gaps and overlaps between genes.

Procedure:

• Clicked preferences under files
• Clicked local setting and new features
• Added SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn into the template notes box
• Calculatted the gap and overlap between some example genes on the questions that matter worksheet
• Calculated the gap by finding the difference between the end of one gene and the start of the next minus one
• Calculated the overlap by finding the difference between the end of one gene and the start of the next plus one

Observations:

• Some of the foward and reverse genes overlap or are too close to be correct (22/24, 24/25, 32/33, and 55/56 are examples of this)

Results:

Some of the genes like 22 (which is reverse) and 24 (forward) are too close and indicate a possible mistake in the auto-annotation. Next lab I will calculate the gap and overlap between genes (especially between forward and reverse genes).