Title: 1/23/19 Annotation Intro

Rationale: 

The purpose of today’s lab was to be introduced to the process of gene annotation, finalize the set preferences for DNA master, and learn about analyzing information given in auto annotation with phage Elesar.

Tools: 

• DNA Master Auto Annotate Function
• FASTA file “Elesar”

Procedure:

• Opened DNA master and uploaded template “SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn” to begin auto annotating Elesar.
• After uploading the template, the genome was auto annotated.
• The ORFs of the genome were examined and identified by using DNA –> Frames –> ORFs

Results

• The majority of the genome contains forward ORFs, with gene number 19 being among some of the longest.
• Auto annotation only revealed approximately 11 reverse ORFs, making up only 16.67% of the genome.

Conclusions:

The genome of Elesar’s lack of reverse ORFs can also indicate a lack of primers that begin coding in the reverse direction. Also, the size of the ORFs, regardless of direction, could have a correlation between the size of proteins produced and/or the number of proteins produced within that gene.

Next Steps:

Need to continue with the familiarization of DNA master so it can be fully utilized to manually annotate genomes. Further practice is needed to begin manually annotating NapoleonB and discovering which proteins are coded within the genome.