Rationale:

To learn how to BLAST genomes and further gain understanding of DNA Master using the genome of the phage Elesar.

Procedure:

1. Opened Elesar and adjusted local settings by inserting“SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn”.
2. Autoannotated Elesar
3. Opened ORFs for Elesar and viewed them

Results:

A greater understanding of DNA Master, specifically auto-annotation and ORFs,  was achieved through practicing with Elesar.

Conclusion:

My understanding of DNA Master was strengthened, which will be useful for further practice annotation on Elesar and the future annotation of NapoleonB’s genome.

Future Plans:

To continue to learn how to use DNA Master in order to annotate the genome of the phage Napoleon B and publish the annotations on PhageDB.