Rationale:

The purpose of today’s lab was to be introduced to the process of gene annotation. Elsar genome was annotated as an example for today’s lab to familiarize with the DNA Master program.

Procedure: 

DNA Master opened and preferences was opened > local settings > new feature. Checked “insert template into notes during annotation” and pasted: SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn. Elesar.fasta was opened and was auto-annotated.

Results:

Conclusions:

At first, all of the information was overwhelming, but once the TA’s cleared the confusion, work was done. The reading frames lines were easy to understand after an explanation of how DNA Master presents the reading frames.

Future plans: 

Learn more about DNA master and to begin annotation the genome of Napoleon B.