January
24
DNA Day 2
23 January 2019 ✷ DNA Master Annotation
Rationale: DNA from Elesar was auto-annotated in order to differentiate the genes within the genome and lay the path for further research in the genome of a phage discovered in 2018.
Procedure
- DNA Master was opened and preferences was opened > local settings > new feature
- checked “insert template into notes during annotation” and pasted “SSC: CP: SCS: ST: BLAST-Start: Gap: LO: RBS: F: SIF-BLAST: SIF-HHPred: SIF-Syn” into box
- Opened Elesar.fasta and auto-annotated it
Results
Conclusion
DNA master is a useful tool in which DNA can be analyzed and genes may be characterized. The processes and the program as a whole are still a bit confusing, but the overall idea of it is logical.
Future plans
This same method will be applied to Napoleon B, a phage isolated in 2018, in order to analyze its genome.