January 24

Beginning An Annotation (1/23/19)

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Rationale:

Insert template and learn more about basics of annotating.

Procedure:

  1. Opened DNA Master.
  2. Changed preference to insert template during auto-annotation.
  3. Auto-annotated Elesar.

Results:

The template was successfully inserted and auto-annotation was successfully performed with the template on Elesar as shown below.

Conclusion:

No difficultly was faced when auto-annotating Elesar. The conceptions learned today in lab were reading frames, how to calculate gaps and overlaps, and when a gap is required. A gap of at least 50 base pairs is required when the promoters are going in opposite directions.

Future plans:

Start analyzing the auto-annotation of Elesar and performing BLAST on Elesar.


Posted January 24, 2019 by Kathryn Adkins in category Kathryn Adkins

About the Author

Kathryn Adkins is currently a freshman attending Baylor University majoring in neuroscience with a minor in biochemistry.  She hopes to one day earn an M.D./Ph.D. and become a pediatric oncologist and cancer researcher. Kathryn volunteers at Cook Children’s Hospital in Fort Worth and is actively involved in AMSA (American Medical Student Association) and BURST (Baylor University Research in Science and Technology).

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