January
24
Beginning An Annotation (1/23/19)
Rationale:
Insert template and learn more about basics of annotating.
Procedure:
- Opened DNA Master.
- Changed preference to insert template during auto-annotation.
- Auto-annotated Elesar.
Results:
The template was successfully inserted and auto-annotation was successfully performed with the template on Elesar as shown below.
Conclusion:
No difficultly was faced when auto-annotating Elesar. The conceptions learned today in lab were reading frames, how to calculate gaps and overlaps, and when a gap is required. A gap of at least 50 base pairs is required when the promoters are going in opposite directions.
Future plans:
Start analyzing the auto-annotation of Elesar and performing BLAST on Elesar.