1.23.19 Introduction to Annotation

Rationale: Today’s procedure was necessary to continue our understanding of DNA Master and gene annotation. Therefore, participating in the lecture, tweaking our DNA Master settings, and working through the QTM worksheet were prudent steps to being introduced to annotation.

Procedure:

• Opened fasta file “Elesar” on DNA master
• Adjusted template to be inserted during autoannotation
• Auto-annotated Elesar genome
• Used DNA–> Frames –> ORFs to identify all Open Reading Frames

Results:

Open reading frames of Elesar were found and briefly examined. Manual annotation of the genome has yet to be learned, so no further progress or understanding was achieved during this lab session.

Conclusion:

Since it was found to be possible to set up the DNA Master program for Open Reading Frame use, it will later be possible to find the Open Reading Frames on NapoleonB. This will be helpful as the genome is being processed and analyzed.

Future Plans:

Skills and lessons learned through the lecture, DNA Master steps, and QTM will later be helpful in analyzing NapoleonB, which will occur at a later date.