1/16/19

Rational:

To auto-annotate Elesar’s genome in order to practice for the annotation of the phage found in class.

Procedure:

• Downloaded the fastA for Elesar phage
• Opened fast file by clicking file open and fastA multi sequence file
• Clicked export and created a sequence from only that entry
• Clicked genome at the very top of the screen and clicked auto-annotate
• Clicked features in the fastA library to see the geneome info
• Clicked DNA and frames to see ORF analysis
• Clicked ORF button to compare potential start codons
• On fastA library clicked genome and profile
• To create ORF map clicked DNA and export map then draw

Conclusion:

The genome of Elesar was annotated. Next lab I will use the steps taken to annotate Elesar to annotate the phage found in class.