Rationale/Purpose:

The purpose of the lab was to download and use DNA Master to Auto Annotate Elesar.

Tools/Procedure:

1. DNA Master was downloaded before the class started.
2. Some preferences in DNA Master were changed.
3. Elesar’s FASTA file was obtained off phagesdb.org.
4. The file was added into DNA Master and auto annotated.

Observations/Results/ Conclusions:

DNA Master was downloaded, and the preferences were changed according to the protocols of the lab. Phage Elesar was used to practice and successfully auto annotated using DNA Master. The ORF’s are viewed in the green and the red sections shown below.

In lab I learnt how to navigate DNA Master specifically on how to Auto Annotate using a FASTA file.  

Future Work:

The next step will be to gain a better understanding of some of the features and to be able to interpret the visual results of the Auto Annotations, and to learn how to annotate other phages.