Rationale:

The purpose of this lab was to familiarize myself with DNA Master with the goal of annotating the genome of the phage NapoleonB.

Procedure/Tools:

• DNA Master

1. The FastA file for the phage Elesar was downloaded.
2. DNA Master was opened and the FastA file for Elesar was selected and opened.
3. An auto-annotation was run for Elesar’s genome.

Results:

The genome of Elesar was auto-annotated revealing 66 genes with 11 reverse genes and 55 forward genes. DNA Master had been previously downloaded and setup.

Conclusion/Future Work:

From the data collected it can be concluded that Elesar has 66 genes, 55 of which are forward and 11 of which are reverse, a greater understanding of DNA Master was also achieved. In the future, I will continue to annotate Elesar in order to become more familiar with DNA Master and the annotation process in order to successfully annotate NapoleonB.