Rationale:

From a computational device, changes were made to the system to accommodate and gain the necessary information for the lab. Also, from the FASTA file with the phage Elesar, the genome of the phage was used for practice to understand the information processed on DNA Master.

Tools:

Computational Device

DNA Master

FASTA File of a phage

Results:

• After presentations, the preferences on DNA Master were changed to provide for easier access to the information needed for the lab. The preferences that were changed were the colors which labeled the different segments of information and some slight changes in the settings.
• After the preferences were changed, a FASTA file containing the genome of the phage “Elesar” was downloaded off of phagesdb.com which was used for practice.
• The file was unable to download under the file tab in finder; however, it did work when moved to the desktop.
• After the file was selected and exported, auto-annotated was selected in which allowed for the open reading frames to be identified.
• In order for open reading frames to be evaluated further, a feature allowing for the 5′ to 3′ was selected.
• Through these steps, more practice was completed in order to become familiar with the program.

Conclusions:

After DNA Master was downloaded, the preferences were changed to allow for better management of the program. Although some difficulty was encountered when the FASTA file was downloaded, it worked when moved to the desktop then moved to the program. After the file was placed in the program, instructions were placed on how to work the program and where certain options were located. Some practice was done with the phage “Elesar” to become familiar with the program for future use.

Future Works:

Next class, the information that is present will be taught and will allow us to understand what is being displayed in order to annotate the phage “NapoleonB.”