DNA Master Setup

Date: 1-16-19

• Rational
  • The rational for this lab was to set up DNA Master for future use and to start with the annotation of phage Elesar.
• Procedure
  1. DNA Master was downloaded and opened.
  2. Preferences were changed for multiple fields. Colors of certain sequences were changed in order to match the rest of the classmates and their annotations.
  3. The option to use PBI server for SEA-PHAGES courses was selected.
  4. A fasta file of a phage (in this case, phage Elesar) was downloaded and opened in DNA Master.
  5. The fasta file was auto-annotated by going to File > Open > FastA Multiple Sequence File and selecting the desired file.
• Results
  • The result of this lab was becoming comfortable with using the program and opening files.
• Conclusion
  • DNA Master can be difficult to get running. Preferences must be checked before working to make sure that everything will run well.
• Future Plans
  • In following labs, the goal will be to more thoroughly go through annotated gene sequences, editing and recording information over time.