Rationale

Today we presented our phage therapy powerpoints, downloaded DNA Master onto our personal computers, and began practicing annotating DNA on the genome of phage Elesar.

Procedure

• Various groups from the classes presented their articles on phage therapy.
• DNA Master was downloaded onto the computers.
• A FastA file was downloaded for Elesar from PhagesDB.
• Elesar was uploaded to DNA Master and was auto-annotated.

Observations/Results

DNA Master revealed that there are 66 ORFs in Elesar. There are 11 reverse ORF of the 66 documented. There are also 55 forward ORFs. Some tasks on DNA Master took a large amount of time to process, however, each task ran smoothly.

Conclusion/Next Steps

I really look forward to learning more about DNA Master and the different discoveries that can be made while using the program. In the coming months, we will dive more into the program and learn more about phage NapoleonB. We also will attempt to improve our presentation skills throughout the semester.