Annotation and Introduction to DNA Master (1/16/19)
Rationale:
Presented scientific article to the class and set up DNA Master on laptop to use for future genome analysis.
Procedure:
- Installed and updated DNAMaster on laptop.
- Downloaded FASTA files for Elesar off phagesdb.org.
- Performed and analyzed auto-annotation of Elesar.
Observations:
Elesar has 66 genes of which 11 have reverse ORFs and 55 had forward ORFs.
Compared to all of Elesar’s other genes, gene 19 had the longest length which was 4824 nucleotides.
Results:
DNA Master was successful downloaded onto laptop. Also, was able to successfully access ORF Analysis of Elesar as shown below.
Conclusion and Next Steps:
Although my laptop screen looks like gibberish to me now, I look forward to learning and using DNA Master to annotate and perform analyses on NapoleanB. Also, I look forward to improving my presentation skills. I hope to become more familiar with DNA Master by annotating and BLAST Elesar.
