Rationale: 

The purpose of today’s lab was to present the phage therapy powerpoint and configure DNA Master and its preferences to begin annotation. FASTA file of phage “Elesar” was loaded and auto-annotated as well.

Tools Used:

• Computer/Laptop
• DNA Master Program

Procedure:

The lab began with presentations which covered an array of topics all focused on the area of phage therapy and the experiments associated with the practice. Once presentations were completed, the classroom discussed various methods to increase our ability to present things scientifically, and how to improve on being a public speaker overall. Once this exercise was complete, the lab was walked through the DNA Master installation process, and the FASTA file for phage Elesar was uploaded and auto annotated.

Observations:

• Several phage therapies, such as phage pVp-1 therapy with Vibrio parahaemolyticus, were successfully tested against animal patients and host to treat human illnesses.
• Enclosing phage used for treatment in liposomes allows the phage to survive longer in the patient’s body, providing a solution to the issue of the immune system of the patient attacking the foreign phage particles.
• The auto annotated results of phage Elesar yielded 66 genes with one RNA and 66 ORFs.
• 

Conclusions:

It can be concluded that the lab has a general knowledge of phage therapy, and what our research means in relation to that. Also, with DNA master installed, it now yields the opportunity to begin to annotate phage NapoleonB, to discover which genes code for which proteins, and the functions behind each of those coded proteins.

Next Steps:

The next steps for this research are to begin manually annotating the genome of Arthrobacterphage NapoleonB to discover the proteins coded by the genes and what the function of those proteins are.