Rationale:

The purpose of today’s lab was to learn how to operate DNA Master as well as present the presentation we made on Monday about the articles pertaining to phage therapy.

Materials:

• Laptop / Desktop Computer
• DNA Mastering Program

Procedure:

Began class with presentations regarding the articles on phage therapy. Then installed the DNA Mastering application on laptop. Updated the application and then downloaded the Elesar FASTA file from the PhagesDB website. Elesar was then auto-annotated by DNA Mastering.

Observations:

The frames in green represent a forward ORF, while the red represents a reverse ORF

Although there were a few problems initially, the FASTA file Elesar was successfully annotated

Results/Next Steps:

DNA Mastering auto-annotated Elesar and resulted in ORF’s. Although most of it is confusing and gibberish on the screen, with enough time and exposure/practice, the class’ genome will be able to be annotated. Hopefully, the lab can began to annotate NapoleonB and start determining functions of the genes.