Purpose: The purpose of this lab was to become familiar with and understand how to use DNA Master, as well as learn how to auto annotate.

Tools/Procedures:

• Tools
  • DNA Master
  • Elesar
  • PhagesDB

1. DNA Master was successfully updated and installed.
2. The Fasta file for Elesar was obtained from PhagesDB and imported to DNA Master. The file was exported and a sequence entry was made.
3. Auto-annotation was performed on Elesar.
4. The auto-annotations were examined and saved.

Results:

• No annotations were started today.
• DNA Master was successfully opened and used.

Conclusions:
DNA Master was successfully installed and the process of learning how to operate the software was begun. The installation and proper set-up took time, and the software had to be reinstalled. After reinstallation and troubleshooting, the program is successfully running.

Future Work:
For future work, the next step will be to learn how to annotate and begin the process.