11/26/18

Rational:

To get an image of the phage found in lysate 8. Also to start DNA extraction by pelleting the phage in lysate 8 and breaking down the free DNA.

Procedure:

• Cleaned lab desk
• Put 10 mL lysate 8 into a tube
• Added 40 ML nuclease mix
• Added 4 mL phage precipitant solution
• Put in shaking incubator for 30 min at 37 C
• Let sit at room temperature for 40 C
• Spun down at 10,000 G for 20 min
• Pulled off teh supernatent
• Froze the remaining liquid and pellet.

Conclusion:

The TEM showed that thr phage had a 106 Mm tail and a 45 Mm head (diameter). Next lab I will do DNA extraction on the phage pellet formed this lab. Nanodrop will also be done on the phage DNA obtained from the DNA extraction.

Fig.4.8 – This image show one of the phage found in lysate 8.