Rationale: Due to the negative results of the plaque assays, modifications were made to the procedure to achieve a high titer.

Procedure:

• Three sets of dilutions to the 10^-4 were made by adding 90 microliters to each tube.
• For one set of dilutions, 7 microliters of the 10^0 lysate were used then diluted.
• For the second set, 14 microliters of the lysate were diluted to the 10^-4
• For the third set, 30 microliters were used.
• Using the 10^-4 dilution from each set, three plaque assays were made.
• Each dilution was combined with 0.5 mL of Arthrobacter and left alone for 10 minutes for infection.
• Then, three sets of cultures were made using 2.0 mL of LB Broth and 22.5 microliters of calcium chloride.
• The infected Arthrobacters of each dilution with a culture then top agar was added and plated.

Results and Analysis:

Conclusion:

Three concentrations of an original lysate were made with dilutions to the 10^-4. The first concentration was 7 microliters of lysate then diluted. The 2X concentration consisted of 14 microliters and 3X concentration consisted of 30 microliters. Using the 10^-4 of each concentration, plaque assays were made using the lysate, Arthrobacter, LB Broth, calcium chloride, and top agar.

Future Plans:

Calculate the titer of each plate then flood in order to get more lysate for DNA extraction