Rationale: Results for the second plaque assay from MEL 2 did not show any plaques so perform a plaque assay for a new plaque picked from Melissa’s plate labeled MEL X

Process:

1. washed table and setup aseptic zone
2. picked plaque and swirled it in 50 µL phage buffer (MEL X plaque lysate)
3. Made LB agar media for 3 plates
   • 6 mL LB broth
   • 68 µL 1 M CaCl2
   • 7.5 mL 2X TA
4. added 50 µL lysate (Mel X plaque) to 0.5 mL arthro and let sit for 15 minutes
5. Poured control plate with ~5mL from the LB agar media
6. Added arthro lysate mixture to  LB agar media
7. Poured plate from tube and let sit for ~15 minutes
8. Incubated for 48 hours at 28 degrees Celsius

Next Steps: Another plaque assay but with alterations to get a higher titer.