11/28/18

Rational:

To do DNA extraction on lysate 8 in order to obtain a sample of DNA from the phage found in lysate 8.

Procedure:

• Cleaned lab desk
• Added 500 ML of sterile water to phage precipitate
• Added 2 mL of DNA clean up resin and mixed
• Put the mixture into two centrifuge tubes and spun at 12,500 G for 3 min
• Pulled off the supernatent
• Added 1 mL of 80% isopropenol and spun at 12,500 G for 3 min
• Pulled off the supernatent
• Repeated the previous two steps two more times
• Added 1 mL of 80% isopropenol
• Put the solution in a column tube and filtered
• Centrifuged the filtered column at 12,000 G for 5 min
• Put the remaining solution in a new centrifuge tube and added 100 ML of 80 C elution buffer
• Centrifuged for 1 min at 12,000 G
• Ran the nanodrop on the sample

Observations:

• DNA concentration- 417.74 ng/ML

Conclution:

The nanodrop results showed the the DNA concentration in lysate 8 was 417.74 ng/ML. As there is not enough time nothing else will be done with this sample however, next I would have done PCR and gel electrophoresis on lysate 8 since it has a high titer.