Rationale: Prepare DNA extraction/PCR by performinf phage precipitation.

Procedure: Lysate obtained and 10mL of lysate was added into a 50mL vial. In the back of the lab, 40µL of nuclease mix was added into the 50mL vial, and this was not done in an aseptic zone. Inverted the vial 10 times and added 4mL of phage precipitant solution. Placed the vial in the incubator at 37 degrees Celcius for 30 minutes, and then incubated the solution at room temperature for 45 minutes. The lysate was then centrifuged in a swinging bucket at 10,000xg for 20 minutes. The supernatant formed from the centrifuge was poured into the sink, but not letting the pellets flow out of the solution. The pellets were poured onto a paper towel, dried, and placed into a microcentrifuge cap labeled ML DNA 11/12/18.

Observations: Experiment performed 11/26 was used to determine titer. Titer calculated to be at 1 x 10^9. Plate from 11/26 experiment showed contaminations, but the phage particles were countable.

Conclusions: DNA extraction will be performed using the products made from 11/28 experiment.