Rationale: Will be running the procedure of pelleting the lysate to obtain the bacteriophage DNA

Procedure:

• Created an aseptic zone to prevent bacterial contamination
• Added 10mL of lysate into a 50mL conical tube
• Pipetted 40μL of nuclease into the tube and inverted the tube around 10 times
• Added in 4mL of PEG and inverted the tube one to two times
• Placed the tube into the shaker for 30 minutes
• Took the tube out of the shaker and allowed to sit in room temperature for 45 minutes
• After the allotted time, moved the tube into a centrifuge and allowed to centrifuge for 20 minutes at 10,000 G
• After centrifuging, decanted the supernatant in the tube and collected the pellet(s)

Observation:

The spot test to confirm the strength of the titer (Was 1 X 10^9)

Conclusion/Next Steps: Will be continuing the next steps of DNA extraction with the hopes of breaking open the capsid of the bacteriophage.