November 28

Transmission Electron Microscopy

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Objectives: Using TEM grid from one week prior, find and photograph novel phages.

Rationale: An important part of classifying the phages is observing their physical characteristics. TEM allows us to observe the phage at a visible level, and record it.

Procedure:

  • Removed the copper TEM grid from Monday, November 19th, from its grid location.
  • Using fine tweezers, placed the copper grid into vacuum tube.
  • Operated the machine to find three separate phages, taking photographs of all three
  • Removed grid from vacuum tube
  • Placed back into used TEM tray

Results:
We now have pictures of our phage.

Interpretations: Its uplifting to finally see the phage, especially in the quantities that we saw it.

Future Plans: DNA extraction next lab period!


Posted November 28, 2018 by Shepard Saabye in category Uncategorized

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