November
28
Transmission Electron Microscopy
Objectives: Using TEM grid from one week prior, find and photograph novel phages.
Rationale: An important part of classifying the phages is observing their physical characteristics. TEM allows us to observe the phage at a visible level, and record it.
Procedure:
- Removed the copper TEM grid from Monday, November 19th, from its grid location.
- Using fine tweezers, placed the copper grid into vacuum tube.
- Operated the machine to find three separate phages, taking photographs of all three
- Removed grid from vacuum tube
- Placed back into used TEM tray
Results:
We now have pictures of our phage.
Interpretations: Its uplifting to finally see the phage, especially in the quantities that we saw it.
Future Plans: DNA extraction next lab period!