Rationale: The purpose of this lab is to web plates to obtain more of a higher titer lysate.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an ethanol burner was lit.
2. Lysate 1 was diluted out to 10^-3.
3. Top agar was made for 7 plates. 14 ml of LB broth and 157.5 ml of CaCl2 were added to a tube.
4. 10 ul of 10^-3 lysate was added to 6 tubes of 0.5 ml of arthrobacter and was allowed to sit for 10 minutes.
5. 17.5 ml of 2x TA was added to the tube and mixed. 4.5 ml of the solution was added to each tube of arthrobacter and poured onto 6 plates labeled LIP 11-26-18 webbed plate. The rest of the solution was poured onto a plate labeled LIP 11-26-18 Control.
6. The plates were allowed to sit for 10 minutes and then inverted and stored in the incubator until the next lab.
7. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• Bubbles on the control plate.

Interpretations/Next Steps:
The procedure was complete. The next step will be to flood the plates to collect a higher titer lysate.

Webbed Plates: