Rationale: Created webbed plates from the team’s lysate (With titer strength of 2.2*10^11) to obtain enough lysate for all the members of the team.

Procedure:

• Aseptic zone to prevent contamination from bacteria
• Obtained the previous lysate and obtained six agar plates
• Added in 10μL lysate into each 0.5mL arthrobacter vial (Six total)
• Obtained a conical vial and pipetted in 14mL LB Broth, 17.5mL 2XTA and 157.5μL CaCl2
• Immediately pipetted 4.5mL into each arthrobacter+lysate vial and then plated
• Allowed to sit for 15 minutes and then moved to incubation

Observations:

The spot test for lysate #5; not the lysate used to web

The spot test for lysate #6; not the lysate used to web

Conclusion/Next Steps: Will be returning in 24 hours to flood the plates and then filter then to obtain a lysate of equal strength tot he 2.2*10^11