11/19/18

Rational:

To make an EM grid for lysate 8 in order to do a TEM and get an image of the phage in lysate 8.

Procedure:

• Put 15 ML of lysate onto a plate
• Put 2 20 ML drops of water onto the plate and 1 drop of uranyl acetate
• Put the EM grid from A9 darks side down on the lysate and waited 5 min
• Put the grid on the first water drop and waited 2.5 min and repeated for the second water drop
• Put the grid on the uranyl acetate for 1 min
• Put the EM grid in A9 after blotting excess moisture

Conclusion:

Next lab I will do TEM using the EM grid from this lab in order to get an image of the phage that are found in lysate 8. I will also start DNA extraction on lysate 8.