Rationale: To prepare a TEM grid in order to view the phages under a transmission electron microscope

Procedures:

• Using a petri dish, a strip of parafilm was set across the dish.
• A drop of lysate (.15 µL) was set on the parafilm.
• The, two drops of water (the same amount as lysate) was added.
• After, one drop of uracil acetate was added onto the strip.
• For five minutes, a copper filter was in the lysate.
• After the five minutes, it was moved to the water for two and a half minutes.
• The previous step was done again in the second drop of water.
• After it has been in the water for its allotted time, the filter was placed in the uracil acetate for one minute.
• The filter was removed and placed into its place.

Results and Analysis:

Conclusion:

To prepare a TEM grid, a drop of lysate was added to the strip of parafilm on the petri dish. Two drops of water and one drop of uracil acetate were then added. For five minutes, the copper filter was placed in the lysate then moved to the two drops of water for two and a half minutes each then moved to the uracil acetate for one minute.

Future Plans:

In order to complete a DNA extraction, 10 mL of lysate is needed. Therefore, a plaque assay will be made then flooded in order to get more lysate.