Rationale: perform a second plaque assay for lysate collected from “mel plaque”, or the singular plaque found on the first plaque assay from Melissa’s soil

Control for first plaque assay of Melissa’s soil

First plaque assay for Melissa’s soil showing one plaque

Process:

1. washed table and setup aseptic zone
2. picked plaque and swirled it in 70 µL phage buffer
3. Made LB agar media for 2 plates
   • 4 mL LB broth
   • 50 µL 1 M CaCl2
   • 5 mL 2X TA
4. added 50 µL lysate (Mel plaque) to 0.5 mL arthro and let sit for 15 minutes
5. Poured control plate with ~5mL from the LB agar media
6. Added arthro lysate mixture to  LB agar media
7. Poured plate from tube and let sit for ~15 minutes
8. Incubated for 48 hours at 28 degrees Celsius

Next Steps: Another plaque assay but with alterations to get a higher titer.