Rationale: To calculate the titer of our plate and create webbed plates to obtain more lysate.  

Procedure: 

1. Created an aseptic zone by cleaning the table with Cidecon and 70% Ethanol, and using a ethanol burner.  
2. The results of the spot test were used to determine the high titer of the plate as well as the amount of lysate to web a plate.  
3. 26.7 uL of 10^-2 lysate dilution was added to 10 uL of Arthrobacter and let to infect for 10 minutes.  
4. Lysate was running low, so added 10 uL of 10^-1 lysate into 90 uL of phage buffer and vortexed.  
5. 12 mL of LB Broth was added to a 50 mL tube.  
6. 135 uL of CaCl2 was added to the tube.  
7. 15 mL of Top Agar was added to the tube.  
8. Using a pipette ,4.5 mL of solution was added to Arthrobacter and lysate solution to the plates.  
9. Remaining solution in 50 mL tube was poured onto the control plate.  
10. The plates remained for 10 minutes until set.  
11. The plates were inverted and incubated until next lab.  

Observations/ Results: 

Spot Test Titer results showed that 10^-4 was the solution to use to calculate for the titer. Titers for 10^-3, 10^-2, 10^-1 created webbed and highly lysed plates.  

High Titer Calculations: 

The value obtained for the amount of lysate to use to create a webbed plate was very small, so we are using the equivalent lysate value for the 10^-2 dilution.   

Next Step/Conclusion: 

Next time, the plates will be flooded, shaken, and filtered to obtain the lysate to plate a higher titer plate.