Rationale: The purpose of this lab is to flood webbed plates to create more of a high titer lysate.

Description of Procedures: 

1. The workstation was cleaned using aseptic technique and an ethanol burner was lit.
2. 6 ml of phage buffer was added to each of the four webbed plates. The plates were placed on the shaker and allowed to shake for one hour.
3. The lysate was collected from the plates and filtered with a 0.22 um syringe filter into a tube labeled LIP 11-20-18 Lysate 3.
4. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• Plates not fully webbed.

Interpretations/Next Steps:
The procedure was complete. However, the plates were not webbed. Due to this the titer most likely will not increase. The next step will be to perform a titer test.

Webbed Plates and Control: