Rationale: Calculate titer from spot titer experiment. Webbed plates made to yield more lysate so each member who contributed to obtaining a high titer lysate could use 10mL of lysate.

Procedure:  Before the experiment was performed, the workspace was cleaned with both Cidecon and 70% Ethanol. Top agar solution made in a 50mL vial using the formula below (6 plates for experiment and one for control):

• 2mL LB Broth (x7)
• 2.5mL 2X TA (x7)
• 22.5µL CaCl2 (x7)
• lysate
  • 10µL of Flooded lysate x8

4.5mL of this solution was added to a test tube containing 0.5mL Arthrobacter phage + 10µL of high titer lysate. This solution was quickly poured on to a plate, sat for 15 minutes and quickly placed in the incubator for 24 hours at 27 degrees Celsius.

Observations: Spot titer experiment performed on 11/16 during open lab results were used to help calculate titer. The formula used below to calculate titer below:

(22pfu/10µL) x (1000µL/1µL) x 10^8 = 2.2 x 10^11

Leaf for ML soil B was obtained to characterize tree. Tree classified as Quercus palustris.

Conclusions: High titer obtained, but the lysate was used to perform webbed plates. The experiment performed on 11/19 will be flooded on 11/20 and titer will have to be recalculated on 11/26 since the new obtained flooded lysate x9 has different phage concentrations.