Rationale: Melissa wanted more lysate from her soil that tested positive for phage so Lily and I washed and enriched more soil

Process:

• Wash table and set up aseptic zone
• Added ~7 mL LB broth to soil (filled to 5 mL mark)
• Shook and vortexed for 15 minutes
• Centrifuged for 10 minutes at 3000 G
• Filtered with syringe filter
• LCG sample enriched and incubated for 48 hours at 28 degrees C
• RSM sample stored as direct in fridge
  • there was not enough arthro for both samples to be enriched

Next steps: Perform plaque assay with enriched sample to check for any plaques