Rationale

Today we will flood webbed plates created on 11/7/18 to create more lysate from Lucy’s phage.

Procedure

• Established an aseptic zone.
• 5 mL of phage buffer was poured onto one previously webbed plate. The process was repeated for another plate The plates were placed on a shaker for 1 hour.
• After 1 hour had passed, the flood lysate was filtered through a 0.22 µm syringe filter and was placed in the fridge for later use.

Observations

The plates that were flooded are shown above. One plate fell off the shaker, causing 5 mL of phage buffer to be added again and the 1 hour long waiting period to be restarted.

Conclusions/Next Steps

8.3 mL of lysate was collected from flooding, which will allow for use in later plaque assays and spot tests in pursuit of a high titer.