Rationale: The purpose of this lab is to flood four webbed plates to collect more lysate.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an ethanol burner was lit.
2. The four plaque assays done before were flooded with 8 ml of phage buffer each.
3. The plates were stored in the refrigerator until the next lab.

Observations:

• The plates were not completely webbed.

Interpretations/Next Steps:
The procedure was complete. The next step will be to collect the lysate and perform a spot titer test to calculate the titer of the lysate.

Flooded Plates: