Shepard Saabye
November 14th, 2018
SEA PHAGE Lab Journal

Results from the Previous Lab: The control plate was contaminated, but interestingly, it was not contaminated when Dr. Adair looked at it the day prior. I have no way to interpret that information. Another plate had some plaques form but due to contamination, Cooper and Michael’s plates were used for a plaque assay 

Objectives and Rationale: Plate Serial Dilutions in order to determine the titer of the lysate and web a plate

Procedure:

• Established Aseptic Zone
• Diluted lysate out to 10^-4 of the original lysate
• Obtained 3 vials of .5 mL Arthrobacter
• Added 10 mL LB Broth, 12.5 mL 2X TA and 112.5 uL CaCl to a single 50 mL tube
• Added 10 uL of 10^-2, 10^-3, and 10^-4 Lysate to their own respective Arthro tubes
• Added 4.5 mL of Agar mixture to each Arthro tube, and plated
• Plated remaining 4.5 mL Agar mixture on the control plate
• Waited 15 min for Top Agar to solidify
• Left plates in the incubator at 24.5 degrees Celsius

Future Plans: Calculate Titer and continue increasing the concentration of the phage in each lysate