Rationale: Filtered the flooded plates (Two of them) to obtain a new lysate (Lysate 6), and prepared serial dilutions to figure out the titer of lysate 5 and 6

Procedure:

• Created an aseptic zone to prevent contamination of the experiment / procedure
• Obtained the flooded plates from refrigeration and obtained a tube top filter
• Utilized the vacuum hose in the hood to filter the lysate(s) through a 22μL filter
  • Named the new lysate Lysate 6
• Created 10^-1, 10^-2 and 10^-3 dilutions for both lysate 5 and 6
  • Created by adding 90μL of PB to a micro-centrifuge tube and adding 10μL of the previous strength lysate
• Obtained 6 vials of 0.5mL arthrobacter and added 10μL of each lysate into each tube respectively
• Obtained a 50mL conical vial and pipetted in 14mL LB Broth, 17.5mL 2xTA and 157.5μL CaCl2
• Immediately pipetted 4.5mL into each arthrobacter + lysate tube and plated
• Allowed the plates (7 total) to settle for 15 minutes and then moved to incubation

Observations:

• For lysate 6, obtained about 10mL total of lysate
• For filtering the flooded plate, used a tube top filter rather than a syringe filter

Next Steps/Conclusion: Will be coming in on Friday to check the status of the plates. If positive, will calculate the titer strength of lysate 5 and 6. If negative, will have to repeat the procedure.