11/14/18

Objective:

To create a webbed plate with all the lysate available from the 4th purification run that can be flooded.

Procedure:

1. the top agar mixture was prepared for 4 plates.
2. 8 ml of LB broth was added to a conical vial
3. 90 μl of CaCl2 was added to the conical vial.
4. Three 0.5 ml of arthrobacter samples were enriched with the extracted phages, the 10^-1 and 10^-2 dilution for 15 minutes.
5. 10 ml of 2X TA was added to the conical vial.
6. 4.5 ml of the TA mixtures was added to each enriched sample.
7. the samples and the mixture were then plated on three agar plates.
8. 4.5 ml of TA mixture was plated on another agar plate for a control plate.
9. after 15 minutes, all the plates were placed inverted in the incubator.

Analysis and Conclusion

this procedure will probably not make a fully webbed plate but it should result in a plate with a lot of plaque. the plate will be flooded and this will give us a lot of lysate.