11/5/18

Rational:

To calculate the titer of the lysate and to take pictures of the phage in the lysate using the TEM microscope.

Procedure:

• Calculated the titer of the lysate
• For the TEM put 20 ML of lysate on a plate
• Put 2 drops of water on the plate and 1 drop of uranyless
• Took an EM grid from A7 and placed it on the lysate drop for 5 min
• Then placed it on the first water drop for 2.5 min and on the second drop for another 2.5 min
• Placed the grid on the uranyless drop for 1 min and then put the sample in the TEM microscope

Observations:

• The titer of the lysate was calculated to be 6.8 * 10^7
• The TEM showed that the tails and heads of the phage were detached

Conclusion:

Since the TEM showed phage that had their tails detached from their heads and a medium titer was used instead of a high titer the TEM will be redone when a high titer lysate is obtained. Next lab we will be flooding the five 10^-2 lysate plates that were prepared by two other group members.

Fig.1.CW – This image shows what could possibly be one of the arthrobacter phage present in the lysate.