Rationale

Due to the webbed produced from 11/5/18, we will make more webbed plates for potential flooding in the future.

Method

• Established an aseptic zone.
• 10 µL of LIP flooded lysate was added to 0.5 mL of Arthrobacter. This was repeated to produce 2 LIP flooded lysate and Arthrobacter mixes.
• 6 mL of LB Broth, 7.5 mL of 2X TA, and 67.5 µL of CaCl2 was combined into a vial.
• 4.5 mL of the second solution was added to a LIP flooded lysate and Arthrobacter mix and was promptly plated. This was repeated for the second plate.
• Remaining TA mix was poured onto a control plate.
• Plates were set aside for 10 minutes and then placed into the incubator.

Observations

The plate shown was produced on 11/5/18 and indicates what was intended on being replicated. No air bubbles formed when pouring the plate.

Conclusions/Next Steps

Next, we will do serial dilutions on the plate created by LIP and LJF. We will also flood the plates for more lysate.