Rationale

Today we will conduct a plaque assay to help Lucy create a high titer lysate.

Procedure

• Established an aseptic zone.
• 30 µL of phage lysate was added to 0.5 mL of Arthrobacter and was set aside.
• 2 mLs of LB Broth, 22.5 µL of CaCl2, and 2.5 mLs of 2X TA were combined in a separate vial.
• The two tubes were combined and poured onto a petri dish. The dish was set aside for 10 minutes and then incubated for 48 hours.

Observations

No air bubbles were observed when pouring, indicating no possible sources of confusion for plaques when assessing the results at a later date.

Conclusions/Next Steps

Next, we will observe the results of the plaque assay and potentially flood the plate.