Rationale: perform plaque assay with plaque 1 because the previous plaque assay had a contaminated control plate

Plaques assay 1 for p1.                                                 

Contaminated control for p1 plaque assay 1.

Process:

1. Added 30 µL of p1 lysate into o.5 mL arthro and let sit for 15 minutes
2. Made LB agar media for 4 plaque assay plates (control, 3 samples)

   1. Reagents	Amount
      LB Broth	8 mL
      2X TA	10 mL
      1M CaCl2	90 µL

3. Poured 5 mL of media into 3 separate tube for sample plates
   1. One for RSM, LCG, and SJ
4.  Poured Arthro and lysate mixture into an LB agar sample tube
5. Poured contents of tube onto a plate
6. Poured the contents of the remaining LB agar media without lysate onto control plate
7. Let solidify for ~15 minutes
8. Incubate for 48 hours at 28 degrees C

Next Steps: calculate titer of lysate and perform another plaque assay to get a higher titer.